FastQCFastQC Report
Sat 31 Dec 2022
SRR1954835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186688
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.5286895783339047No Hit
GAATCTATCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC5660.30317963661295855No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG5090.27264741172437434No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC4130.22122471717518No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.1462332876242715No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2650.14194806307850533No Hit
GCTGTCACTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC2200.11784367500857044Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)