FastQCFastQC Report
Sat 31 Dec 2022
SRR1954834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889603
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC80550.9054600760114342No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG74250.8346419695077467No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC54650.6143189714962742No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42760.4806638466821717No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC37950.4265947844150705TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT27590.31013834260900647Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC22140.24887505999867357TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT20030.22515661480458138No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT19630.2206602270900615No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC19580.22009817862574652No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT18750.21076817411811785No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA14900.16749044236586433No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC14340.16119549956553653TruSeq Adapter, Index 14 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC12830.14422163594322412No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG12770.14354717778604614No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC10450.11746812904183102No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC10070.11319656071303716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)