FastQCFastQC Report
Sat 31 Dec 2022
SRR1954734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881805
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73010.8279608303423093No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC46290.5249459914606971No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG32820.37219113069215987No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT32530.36890242173723214Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC27900.3163964822154558No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC24180.27421028458672836No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC21500.24381807769291397No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT16520.1873430066738111No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.1415278888189566No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC12280.13925981367762713No Hit
GGGCTTAACTTTCTCATGAATTTGCCTGAGGTTTCCAAACCCTTCACATTT11790.13370302958137004No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC11660.1322287807395059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11340.12859986051337882No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT10100.11453779463713634No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC9910.11238312325287338No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC9790.11102227816807571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)