FastQCFastQC Report
Sat 31 Dec 2022
SRR1954708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526649
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63241.2007997736632938No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC53531.0164265003826076No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG50550.9598423238247866No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC36650.6959094197463587No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC31200.592424935773162No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT27680.5255872507115745No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC19490.3700757050711195No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT16260.30874453383562867No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC15120.28709823810545543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.2758953306661553No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC13990.265641822162389No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT13170.25007167961963284No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT12830.2436157668580022No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA12370.23488129665109023No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC11600.22026055304386793No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.19614582008130652No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG9480.18000603817723No Hit
GAATATGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC8760.16633469350554164No Hit
ACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC8620.1636763764860467No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC7080.13443488927160216No Hit
AAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC6650.1262700584260105No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC6450.12247246268387484No Hit
GCTGACTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC5750.10918087758640005No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.10177556588923553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)