FastQCFastQC Report
Sat 31 Dec 2022
SRR1954699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251544
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.6305059949750341No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.18803867315459719No Hit
GAATCTATCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC4600.18287059122857235No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG3800.15106701014534235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.1272143243329199No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC3070.12204624240689502No Hit
CTGTCTATTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT2520.10018128041217442Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)