FastQCFastQC Report
Sat 31 Dec 2022
SRR1954694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310571
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.8573362297807597No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC36170.2759865737911185RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG27130.20700900599814892No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC20290.15481801443798163No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC13800.1052976145512147RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC13410.10232181240085428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)