FastQCFastQC Report
Sat 31 Dec 2022
SRR1954614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332698
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC38141.1463850098287336No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG33050.9933934078353341No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32960.9906882518079461No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC23900.7183692117175337No Hit
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT11130.334537628720341No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC10010.30087346482395444No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT9040.27171789430654825No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC8970.2696138840630241TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT8130.24436576114073424No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA7080.2128056074878719No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT6970.20949930567661962TruSeq Adapter, Index 14 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.17643628756409716No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG5790.174031704428641No Hit
GAATATGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC5780.17373113153670897No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC5110.15359274777726348TruSeq Adapter, Index 15 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC4110.12353545858406123No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3780.11361655315030449No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC3670.11031025133905223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.10850681398746011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)