FastQCFastQC Report
Sat 31 Dec 2022
SRR1954536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836614
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116271.3897687583521194No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG39020.46640386127891714No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC25310.3025290038177702No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC20860.24933840456889317No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18020.21539204459882336No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC10710.12801602650684785Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC9650.11534590623632883Illumina Single End Adapter 1 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)