FastQCFastQC Report
Sat 31 Dec 2022
SRR1954469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479983
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG72301.5063033482435837No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC49601.033369931851753No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC48091.0019104843296534No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.6821074913069838No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC12770.266051089309413TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT12180.2537589872974668No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT11310.23563334534764774No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC10760.22417460618396903No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA9770.20354887568934735No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5280.11000389597131566No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT5200.10833717027478056No Hit
GAATCTATCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC4890.10187860820070711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)