FastQCFastQC Report
Sat 31 Dec 2022
SRR1954360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911146
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292773.2132062260054917No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG28270.3102686067874962No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC24330.26702636021010906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18310.20095571950049718No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC14790.16232305250750154No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC11430.12544641583236935Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)