FastQCFastQC Report
Sat 31 Dec 2022
SRR1954305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851675
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.21698417823700356No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13760.16156397686911086No Hit
GAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11490.13491061731294215TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC11450.13444095458948543TruSeq Adapter, Index 13 (95% over 23bp)
AAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT9260.10872692048023012TruSeq Adapter, Index 8 (95% over 21bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8580.10074265418146594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)