FastQCFastQC Report
Sat 31 Dec 2022
SRR1954281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049633
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.19540163085573717No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC13450.12814002608530792TruSeq Adapter, Index 16 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13050.12432917029094931No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT11510.1096573754826687No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT10750.10241674947338737TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)