FastQCFastQC Report
Sat 31 Dec 2022
SRR1954219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542266
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.4291251894826524No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12070.2225844880556775No Hit
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC7730.14254996625272467RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT7640.1408902641876865TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC5800.10695857752468346Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)