FastQCFastQC Report
Mon 20 Mar 2023
SRR7868837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21288359
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2282101.0719943232825038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500790.23524124146910527No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG433640.20369818077569998No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG389090.18277125071030606No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAA264540.1242650971829252No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC238390.11198138851378822No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGC215340.10115387475380323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGG51050.020.8022422
CCCGTCG54200.019.8387221
CGGTGGC88100.019.0742191
CGGCATG59700.017.7885176
CGTCGGC63650.016.7422963
TGGCGCG101600.016.370084
CGACCGT7450.016.1470761
GGCGCGT107550.015.4818935
GGTGGCG109600.015.411832
GTGGCGC109550.015.283653
GCTCTAG74300.014.46563218
GTCGGCA76750.014.1978224
GCCCCGA122150.014.19135136
GTTATCC77100.014.10887735
CCCCGAG124050.014.07858537
CGAGCCC125400.013.67557433
TCGTTCG35750.013.66191837
GACCGTT9550.013.3671772
GCGCGTG125050.013.3599926
GTATTAG81050.013.28388712
Produced by FastQC (version 0.11.5)