FastQCFastQC Report
Mon 20 Mar 2023
SRR7868836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27292559
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2081990.7628416228760374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2021170.7405571606532022No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT1878460.6882681832802853No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC1205720.44177608995917167No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC750410.27495039948434297No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA558220.20453193854046448No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCG278490.10203880112524444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAG358650.021.5533391
GCGGGTC245300.021.06881537
AGCGGGT447300.018.59919237
CGTTATG38800.018.02343628
GCGTTAT38700.017.87895627
AGAGCGT27050.017.780469
GTCGGAT22500.017.5931781
CGGAGCG476100.017.48126434
CCCCAGT454750.017.3877032
GTCAAAC484850.017.3483287
CAGTCAA470450.017.2255085
GTCCCCG494900.016.92310729
AGCGTTA41550.016.7423626
CCGGAGC500300.016.73510633
AACTCCC501400.016.694211
ACTGTCC503750.016.6740326
CCCAGTC483750.016.6404783
GATCGGA25200.016.4409262
CCAGTCA493300.016.3904114
CCCGGAG510250.016.33600432
Produced by FastQC (version 0.11.5)