FastQCFastQC Report
Mon 20 Mar 2023
SRR7868835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27938723
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1601950.5733798212609789No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC782940.280234712230763No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC407140.14572605913305345No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC400750.1434389109337603No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCA309600.11081394092349889No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA294720.1054879995767881No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292530.10470414127374397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC241850.027.200311
CGCAAAA56250.021.8765811
ACTAAAT90000.019.7382681
TGGCGCG362500.018.266084
GTGGCGC365100.018.1309683
GGTGGCG369600.017.9561042
CGCAAGT66250.017.8172746
GGCGCGT381700.017.420265
AGCGCAA68700.017.2890034
TGCAGCG70900.017.0430471
GCGCGTG403100.016.5370466
CAGCGCA87650.016.2318083
CGTGCCT417500.015.9166099
CGCGTGC419300.015.8657887
GCGTGCC420050.015.8374888
CGTGTAG25150.015.66719836
GTAGTCC434200.015.24426516
GCAAGTA77950.015.2127867
TAGTCCC436600.015.13493517
TGTAGTC437900.015.12398915
Produced by FastQC (version 0.11.5)