FastQCFastQC Report
Mon 20 Mar 2023
SRR7868833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26586396
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1524480.5734060381858451No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC975170.36679285150194857No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930460.3499759801967894No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC273980.10305270409723831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC271550.022.6580561
CGCAAAA41800.016.8666761
TGGCGCG410350.015.0858784
GTGGCGC411700.015.0813763
GGCGCGT417900.014.8358845
GGTGGCG423200.014.7637532
CGTGTAG33950.014.54858536
TACCGTA82450.014.2257669
CGTGCCT438000.014.1666129
TAGTCCC444950.013.89462917
GCGCGTG450500.013.8650256
GTAGTCC446900.013.86310816
CTACCGT85250.013.7800978
TGTAGTC450950.013.72642515
CCGTATG85900.013.69756711
CGTATGG86050.013.69480212
CGCGTGC458400.013.5601217
GCGTGCC460300.013.5444668
CCTGTAG461300.013.5107913
ACTACCG87650.013.4870777
Produced by FastQC (version 0.11.5)