FastQCFastQC Report
Mon 20 Mar 2023
SRR7868832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26559810
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7294172.7463185918875173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2637280.9929589104741336No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1809590.681326410091036No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC1244210.46845591139394444No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC660100.2485334044181792No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC409520.1541878499883847No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT381150.14350629767306317No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA342180.12883375295229899No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA318650.11997450282965126No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGC279330.10517018005776398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC259450.028.7865261
CGTGTAG85000.020.89320436
TGCAGCG73600.019.284541
AGCGCAA73600.019.1046564
CGCAAGT74150.018.7141066
ACTAAAT91500.018.4646661
CAGCGCA85500.018.0468983
ACGTGTA101350.017.50444835
GTGTAGA102000.017.46547937
GGTGGCG449800.016.8318822
GCGCAAG84700.016.8199185
GCAGCGC86550.016.7596722
CGACCGT28550.016.4633941
GTGGCGC461950.016.3325823
TGGCGCG463200.016.2485944
AACGTGT108850.016.17941934
GTAGGTC87350.015.96920311
GCAAGTA89100.015.7591927
GGCGCGT481350.015.6440775
CTAAATA166650.015.3681841
Produced by FastQC (version 0.11.5)