FastQCFastQC Report
Mon 20 Mar 2023
SRR7868831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26758619
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8432093.1511678536175576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100980.7851601011248002No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1868610.6983207915176789No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC1189310.4444586620856629No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC424550.1586591595029624No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT408490.15265735500027114No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC267680.10003505786303844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC279800.027.4142571
CGTGTAG29800.018.06457736
GGTGGCG469850.016.5587252
GTGGCGC476500.016.3579623
TGGCGCG478800.016.2329544
AGCGCAA64450.016.190314
ACTAAAT70250.015.9370781
CGCAAAA46950.015.7266771
GGCGCGT497150.015.6789455
CGCAAGT65800.015.6621686
CAGCGCA76250.015.0436253
TGCAGCG70150.014.9837441
GCGCGTG521550.014.9457616
CGCGTGC531900.014.601267
GCGCAAG72400.014.5407395
ACGTGTA37300.014.5315135
CGTGCCT542850.014.344419
GCGTGCC545300.014.3103098
CCGTCAT66900.014.0243091
GTAGTCC558550.013.94681716
Produced by FastQC (version 0.11.5)