FastQCFastQC Report
Mon 20 Mar 2023
SRR7868829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21840260
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7445373.409011614330599No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1983530.9081988950680989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1382610.6330556504363959No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC658640.3015715014381697No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC625160.28624201360240215No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT616770.2824004842433195No Hit
CACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG578310.26479080377248254No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC551560.252542781084108No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA450150.20611018366997463No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC407990.18680638417308218No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT268070.12274121278775985No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC238410.10916078837889293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC102950.026.27194237
CTGACGG118300.022.94137836
GCGGGTC92900.018.53980337
GCCCCAG146200.018.0793081
AGCGGGT150000.018.06830837
CACTCCC134950.017.707381
CGGAGCG158550.017.32692134
ACCGACG7850.017.20379837
GCGTCAG31550.016.82882137
CGTTATG21200.016.58012828
GTCCCCG166600.016.4567629
GCGATTG31200.016.2474783
CAATCGG15600.016.2465869
ACGTTCG16400.016.2428026
GTCAAAC171300.016.2206337
GCGTTAT22100.016.1560527
CGATTGA31350.016.0504654
TCGACCG11250.015.95105328
CCGGAGC173100.015.88096833
AGCGTTA22150.015.78549626
Produced by FastQC (version 0.11.5)