FastQCFastQC Report
Mon 20 Mar 2023
SRR7868829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21840260
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1879150.8604064237330508No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1012990.4638177384335168No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC814870.3731045326383477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545870.24993750074403875No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC437120.20014413747821685No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA372000.17032764261963915No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT233840.10706832244671079No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG227280.10406469520051502No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC221770.10154183146171337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC172300.025.7433091
CGTGTAG107200.018.20614436
CGCAAAA52650.017.7138841
CGACCGT22750.016.511871
ACGTGTA121950.015.95868935
GTGTAGA120750.015.94861337
ACCGTTG24050.015.5390133
CTAAATA118200.015.4831911
AACGTGT127350.015.20939734
GACCGTT24850.015.1878012
GGTGGCG297450.015.0954812
ACTAAAT61450.015.0868111
CTACCGT123600.014.77226458
ACTACCG124300.014.7486377
TACCGTA123650.014.736479
GTGGCGC305900.014.6178783
TGGCGCG306500.014.5951984
TAAATAC125500.014.5795372
ACCGTAT127900.014.08772110
CGCAAGT40200.014.0823966
Produced by FastQC (version 0.11.5)