FastQCFastQC Report
Mon 20 Mar 2023
SRR7868827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8065218
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399100.4948409330039188No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG161750.20055254551085913No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC134840.16718704937671863No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCA104010.12896117625090853No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGC93260.11563233628650832No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATA92930.11522317189690348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA20000.022.020071
ACACGTC6250.019.53553214
CGGTGGC38050.018.5772081
GATCGGA7150.017.0779952
CGTCAGA7750.015.9926717
CCGTCAT26350.015.66015151
CGACCGT14850.015.3267611
GACCGTT14650.015.0282472
ACGTCAG8500.014.79946316
CTATTAG20000.014.70760311
CGTGTAG10300.014.54774536
TAACCCC28350.014.421359
ACCGTTG15150.014.4097883
TTAACCC29100.013.9860068
GTAAGAT21400.013.91823724
ATCGGAA8800.013.8755283
TAGTAAG21100.013.852921522
ACTACCG25000.013.6896727
TGCAAGC21500.013.68064437
TTAGCTC22400.013.46173114
Produced by FastQC (version 0.11.5)