FastQCFastQC Report
Mon 20 Mar 2023
SRR6503042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96146424
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24363762.5340266425301476No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8514460.8855721976721673No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6080730.6324447386623552No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA4949390.5147762957881824No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4196940.4365154548025624No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1532340.15937566227112096No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTCCGTTGATA1493190.1553037479584264No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1411350.14679173091242584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT1231440.1280796465191467No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1076160.11192927986588457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2247950.0141.418621
TAGCGAC2256500.0140.02682
GATAGAC1755350.0139.611221
GATGTAC2659800.0138.67612
TAAGGTC2729850.0138.347432
AACAGGC5472050.0137.810072
GAACAGG5412750.0137.321
GGCTAAC1905450.0137.206772
TATCAGC1490900.0136.921841
AGAGATC2649450.0136.904112
TGGAACA2972500.0136.209661
ATAGACA1810850.0136.072622
CTAAGGT2744650.0135.84791
ATCCGTC2389500.0135.796142
ATCAGCA1489450.0135.358082
CTAACGA3044900.0135.355262
ATGAATC1379400.0135.16452
GTGCGAA1651950.0134.893652
GGCTTCA3346150.0134.617692
AGATGTA2748100.0134.556521
Produced by FastQC (version 0.11.5)