FastQCFastQC Report
Mon 20 Mar 2023
SRR6503041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110546024
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15226191.3773620659572523No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4699200.4250899154907643No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG1712480.15491104410955567Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTCG28437250.0143.3771
ACTTCGA28326200.0143.310792
CTTCGAA10134650.0141.455733
CTTCGAT14791050.0140.733083
TTCGATA4981850.0140.569994
ACGTGAT3109400.0140.531172
AACGTGA3092200.0140.512041
ATGCCTA2619250.0140.374511
AACATCG3524800.0140.026932
TTCGAAT5325500.0140.014224
CATCAAG2834300.0139.979861
CTTCGAG3966950.0139.521093
TTCGATT8040200.0139.167334
AAGGTAC2260650.0139.100391
CAAGCTA1916550.0139.05192
AGGTACA2271800.0138.473652
AGAGTCA1620100.0138.279131
CTCAATG2335950.0137.803151
ATCAAGT2877700.0137.780242
GAGTCAA1623000.0137.753072
Produced by FastQC (version 0.11.5)