FastQCFastQC Report
Mon 20 Mar 2023
SRR6503039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163357662
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40163492.458622969273397No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27489331.6827695538394767No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23957141.4665452300608954No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4437550.27164627270436814No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2326850.14243898764907642No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2054190.12574800440030784No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1992990.12200162365203293No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1891130.11576622589027995No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1786600.10936738308607771Illumina Single End PCR Primer 1 (100% over 21bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1755180.10744399610714311No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1678990.10277999693702766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT3316900.0140.94821
CTCAATG3547100.0140.604541
AACGTGA2398550.0139.94531
ATCCGTC3380850.0138.926482
ACGTGAT2458950.0138.02962
GATAGAC1519100.0136.474121
ATGCCTA790950.0134.697851
TGGAACA2668650.0134.459461
CAATGGA3788250.0134.28291
CCGAAGT1339300.0134.238021
ATAGACA1556800.0133.845112
ATGAATC1049750.0133.608172
AGAGTCA967150.0133.530271
TATCAGC1023150.0133.193631
ATCCTGT1693650.0132.58271
CTGATCA918800.0132.474173
CGAACTT618950.0132.104771
AGTTAGC742450.0131.926622
GGCTTCA1844950.0131.777252
ACGCTTA713400.0130.929412
Produced by FastQC (version 0.11.5)