FastQCFastQC Report
Mon 20 Mar 2023
SRR6503038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124637438
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31262442.508270428344331No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19534671.567319604242828No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8099370.6498344422002641No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1933760.15515081431632122No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1738710.13950142332033494No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1442440.11573087694565737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGAC4727100.0140.983435
ATAGCGA1217450.0140.575241
AACATCG2046800.0140.032212
CAAGCTA1633400.0139.751452
CAGATTC2520050.0139.458252
AACGTGA2455100.0139.309971
GAGTCAA1470900.0139.176532
AGAGTCA1474400.0139.17261
ATTGGCT5794800.0139.007521
TAGCGAC1234150.0138.979282
ACGTGAT2479050.0138.838622
AGTGACT2903800.0138.788766
GTCACTA1520700.0138.479352
ACAAGCT1650450.0138.14491
ACCTTAC2383750.0138.088612
ATCAGCA1188700.0137.73632
TATCAGC1188900.0137.722841
CTAAGTG5429350.0137.324483
ATGAATC1006450.0137.226582
TAAGTGA4663800.0137.091664
Produced by FastQC (version 0.11.5)