FastQCFastQC Report
Mon 20 Mar 2023
SRR6503038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124637438
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2984305223.943890759371993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA39552523.1734060515589224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22124101.7750766025854927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10668390.8559538908365558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA9471310.75990891276183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7684010.6165089818357787No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5134650.41196690836985916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3984700.3197032981374344No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3799820.30486987384962133No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3414020.27391609253072097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA3248230.26061431076591934No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3063640.2458041539653599No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2868070.2301130419577463No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2832680.22727360618564704No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2694450.21618303803709443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2663210.21367656803086726No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2621600.2103380847735333No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2450190.19658539515229767No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2365390.18978166094845433No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2340770.1878063315133291No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2280110.18293941504157044No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2200090.17651919321383996No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2108590.1691778998217213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAA2056270.16498012419029345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2046640.16420748314804096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA2039480.16363301691101834No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2001260.16056652255640877No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1997580.16027126616643067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1986920.15941598542807017No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1970100.15806647116735503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1946800.15619704891559147TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1933650.15514198871770776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA1863440.14950884982087004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1862630.14944386132198897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1751680.14054204162957842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1726730.13854023539861274No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1685100.13520014748698542No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1652560.13258937495168988No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1650230.13240243272651353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1642860.1318111176194106No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1629170.13071273175560622No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1601250.12847263436207668No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1471430.11805682334388164No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1436680.1152687365091699No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1436450.11525028298479625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA1426070.1144174674065428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1410480.11316663938486925No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1401350.11243411469995075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1352820.10854042105711448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1348110.10816252497102836No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1258240.10095201090381849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT202037200.058.2630651
AGTGGTA209853900.056.0968135
AGCAGTG210118250.055.979282
GTATCAA211035700.055.7934729
GGTATCA214737600.054.7758528
CAGTGGT215904900.054.5054554
GCAGTGG217262950.054.1540343
TGGTATC218599500.053.8102467
GTGGTAT218897050.053.782586
GGGGAAA5069250.021.3963325-29
TGGTATA686250.021.1186337
GGTATAA692200.020.8957318
GTTGTAT685300.020.790896
TTGTATC686950.020.7093357
GGGGGAA2349550.020.17781625-29
TGGGGAA4544500.020.16174125-29
TGTATCA718650.019.9162778
CAGTTGT749800.018.9860944
TGGGACT1542850.018.89306425-29
GCAGTTG756800.018.8486843
Produced by FastQC (version 0.11.5)