FastQCFastQC Report
Mon 20 Mar 2023
SRR6503036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119267270
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11876430.9957828329599563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5222880.437913939004389No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2475990.207600123655048No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2417530.20269852743338554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3439850.0144.667051
AGTCACT5066250.0144.432251
AACGTGA3614300.0144.088731
CGAACTT2302450.0142.732121
GAGCTGA3026000.0142.696041
GTCACTA5113850.0142.532262
ATGCCTA1732250.0142.510531
CGCATAC2495700.0142.302981
CCGAAGT2008250.0142.134721
ACGTGAT3672900.0141.951892
AACATCG6183850.0141.950492
AGAGTCA1572750.0141.916181
CAAGACT3000650.0141.702441
ACATCGT3156350.0141.087633
ACGCTTA2084400.0140.944872
ACATCGA2087250.0140.306733
AGTGGTC2312600.0140.012941
AAGACTA3040800.0139.778962
AGCTGAA3091150.0139.567722
AGATCGC2118600.0139.420721
Produced by FastQC (version 0.11.5)