FastQCFastQC Report
Mon 20 Mar 2023
SRR6503035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100656681
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2375933423.604328857217137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20456142.032268479029226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14347771.4254165602778022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA9307800.9247076207489893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT7779710.7728955418269752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6385870.6344208786300037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5235400.5201244416155546No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTAAGTAAGAGCACACGGG4954400.4922077651258936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC4716460.4685689964285629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC4689550.46589555242736447No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3256700.32354533923088524No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3139400.3118918653795072No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2404610.23889224004912304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2400610.23849484963645878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2220180.22056956159720786No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2118540.21047187121141017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2101450.20877402067330234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA2087430.2073811672769143No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2022360.2009166187388992No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1974390.19615091421502362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1778300.1766698427101923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAGTAAGAGCACACGGGG1753150.17417125049056606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1626220.1615610592206989No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1616950.1606401069393496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTAAAAAAAAACCAACC1554930.154478568590991No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1539510.15294662855017044No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1492950.14832100414675903No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1491860.14821271525930801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1393290.13842002201523018Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1364780.135587621848966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1331490.13228034013956808No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1330880.13221973810163679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1326490.13178360212373783No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1315010.13064309163939153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1267510.125924080489004TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1255450.12472594839482141No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1206740.11988672664460295No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1186280.11785407468382551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAAACCAA1173890.1166231578805981No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1151260.11437492162095034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG1127830.11204720727876971No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1126290.11189421196989398No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1096890.108973392436812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1088800.10816967032719865No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1047540.10407058822056729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1037880.10311089037398323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1022760.1016087546141125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT155077200.064.407761
GTATCAA160776500.062.050499
AGCAGTG161378800.061.902362
AGTGGTA160442550.061.8541035
GGTATCA163811950.060.8785868
GGATCAA1224000.060.3846939
GCAGTGG165527650.060.3299753
CAGTGGT164673300.060.248934
TGGTATC166897700.059.742837
GTGGTAT167007300.059.660246
CAGTGGG1338600.054.4518974
GTGGGAT1372000.053.5823336
GGGATCA1630250.045.270968
AGTGGGA1707200.042.665615
AAGCTGT1428500.029.7645131
TGTGGTA1478550.027.6748835
AGCTGTG1731600.024.5494292
TGGGATC3275750.022.5103247
GTAAGTA1048100.022.3379630-34
TCCCGAG1017200.021.854616125-129
Produced by FastQC (version 0.11.5)