FastQCFastQC Report
Mon 20 Mar 2023
SRR6503033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142135964
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4369630.30742606424367025No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3388470.23839638502750787No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1735900.12212954069808821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1673000.0144.146591
CCTAATC5667000.0143.575071
GTCGTAG5933300.0143.531191
GATAGAC1409600.0143.256671
CCGACAA5641400.0142.777821
GTCTGTC9256150.0142.705661
AGAGATC8936750.0142.403722
ATGAATC3804650.0142.239582
TAGCGAC1703650.0142.138992
GTACGCA1100700.0142.132351
TTCACGC2797600.0142.01821
TATCAGC1443700.0141.896671
ATCCGTC3922250.0141.890082
GCGAGTA2113600.0141.550171
TCGTAGA6008800.0141.512152
CTGTCAA3207850.0141.4623
GATGTAC3985450.0141.432022
TCACGCA2818900.0141.313342
TCTGTCA9382250.0141.190642
CTGTCAT4684650.0141.127123
Produced by FastQC (version 0.11.5)