FastQCFastQC Report
Mon 20 Mar 2023
SRR6503033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142135964
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1482490310.430085801507632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG125284148.81438704703899No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA70075874.930199790955089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA44512193.1316627226027043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA13493300.9493234238732149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12316420.866523830661183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA11279990.7936056211642537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA9677280.6808466856424881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT7668630.5395277721548363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7587290.533805082575723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA5899620.4150687717571606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4964870.34930427600997593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4928800.34676656500532127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA4528570.3186083150637371No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC4454110.3133696690585642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC4294450.3021367625156431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA4167090.2931763279840984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA3598220.2531533820673281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAA2875850.20233091745872284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC2609560.18359603907143446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA2372740.16693452756263713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAAAAAAAAAAAAAAA2285180.16077422882220013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA2028890.1427429021412202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA1932640.13597121696800116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT1819940.1280421892379046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1814170.12763623990336465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1740240.12243488213862608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1702780.11979937744679453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAAAAAAAAAAAAAAAAAA1665920.1172060858573415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGGAAAAAAAAAAAAAAAAAAA1635580.11507150998040158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAAAAAAAAAAAAAAAA1580040.1111639838035643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAGAAAAAAAAAAAAAAA1549470.1090132262373793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA1491880.10496147196074879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA1476720.10389488757398516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1446350.1017582010419263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT145511300.0103.829741
AGCAGTG149794250.0100.908272
AGTGGTA149191300.0100.595195
GTATCAA150037400.0100.577569
GGTATCA151266600.099.701938
CAGTGGT151074500.099.327814
GCAGTGG152176250.099.3003543
TGGTATC153005700.098.552547
GTGGTAT153177400.098.3898856
GTGGGAT1473050.080.013056
GGATCAA1512750.078.186489
CAGTGGG1508350.077.592414
GGGATCA1877050.062.854788
AAGCTGT1198750.055.9805681
AGTGGGA2611700.044.787365
AGCTGTG1518100.044.180422
TGTGGTA1499850.043.1557855
TGGGATC3090900.038.1457
CTGTGGT2136100.030.2848764
GCTGTGG2295250.029.1927283
Produced by FastQC (version 0.11.5)