FastQCFastQC Report
Mon 20 Mar 2023
SRR6503031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101302941
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9668580.9544224387325537No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5844320.57691513615582No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2562880.25299166783321725No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2112100.20849345331445018No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1151060.11362552642968185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3501150.0143.579861
GCGAGTA6786650.0143.397431
GATAGAC3447750.0143.22141
TAGCGAC3530250.0142.937332
CTAACGA6838300.0142.556322
GCTAACG6856550.0142.478331
TATCAGC2549950.0142.4011
GCCACAT4809450.0142.072651
GTACGCA1584350.0141.902081
TAACGAT3737400.0141.567033
AGCGACT1723350.0141.510253
ATGAATC2945550.0141.500522
GTGCGAA3681400.0141.419542
ATAGACA3499400.0141.39892
ATCAGCA2563400.0141.374052
CGAGTAA6870900.0141.362612
AGTTAGC1509150.0140.650332
TACGCAA1595050.0140.550782
GGTGCGA3720400.0140.067641
CTCGGTA3030150.0139.896072
Produced by FastQC (version 0.11.5)