FastQCFastQC Report
Mon 20 Mar 2023
SRR6503028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130035358
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2597642719.976433640456467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA78012355.999318277725663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA35466982.7274873961588204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33867652.604495463456947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA27673662.1281642489883406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8847620.6804010952159643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA6766250.5203392449613589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6612640.5085263040533945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA4862130.37390830269410263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA4779840.3675800238885796No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3930070.3022308747748439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3009930.23147012061134942No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2952090.22702209963539302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2783320.21404332197093653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2781740.21392181655700135No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2613020.20094688400058083No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2489540.19145100519506394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA2441120.18772740257307555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2385740.1834685609124866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2142570.16476826249057583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA2030120.1561206145177837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1989510.15299761777100657No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1934410.14876030871541876No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1805860.13887453595505925No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1745990.13427040359284434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1732190.13320915377492945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1724330.13260470279168224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGGAAAAAAAAAAAAAAAAAAA1699290.1306790726872917No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1680180.12920947239596173No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1649060.12681627715440288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1591810.12241362845327039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1587660.12209448448628872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1585920.12196067472663857No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1539460.11838780033965837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA1524630.11724734129620346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1496290.11506793406144196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1481540.11393362719084452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1467700.11286930128650086No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1467150.11282700509810571No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1345790.10349415887331197No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1329450.10223757756717215No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1318370.1013855016264115No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1305370.10038577353707134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT186850800.068.4482651
AACCGAG213450.066.432411
AGCAGTG194275250.065.7857362
AGTGGTA193677000.065.556075
GTATCAA194766250.065.48099
GGTATCA198818550.064.0863348
GCAGTGG201317600.063.4529343
CAGTGGT200180100.063.4314424
GTGGTAT203891450.062.48486
ACCGAGA223000.062.479132
TGGTATC203952250.062.4728627
GGATCAA1480050.056.9234549
CAGTGGG1835600.046.009964
GTGGGAT1852800.045.377566
GGGATCA1928800.043.612518
AGTGGGA2262600.037.0119675
CGAAGTA76600.027.5563852
AAGCTGT1699500.026.5045471
CGAGATT158850.023.3860764
TGGGGAA12005900.023.3666825-29
Produced by FastQC (version 0.11.5)