FastQCFastQC Report
Mon 20 Mar 2023
SRR6503025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109222668
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1361382912.464289006380982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32810653.004014697754865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15690331.4365452050667724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA14644281.3407729611585757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA12124941.1101120510991362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7507770.6873820368497133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3328290.3047252059435135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3154470.2888109270504178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3034670.2778425079306797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2857420.2616141916621191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2753400.2520905275816921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2456450.22490294780200754No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2120180.1941153827152437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2107170.19292423803454425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1879450.17207508609842784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1694010.15509692548436924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1676800.153521245241876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1607310.14715901281591107No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1570790.14381538455002765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1568280.14358557877381278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1444290.13223353965314233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1301660.11917489508679645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1167850.1069237752002176No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1102350.10092685155795682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT111020700.079.781821
GTATCAA115361100.076.568989
AGCAGTG115793600.076.4554442
AGTGGTA115150250.076.33215
GGTATCA117617050.075.029618
CAGTGGT118596050.074.112854
GCAGTGG119663600.073.945383
TGGTATC120875100.073.009617
GTGGTAT120890500.072.9841166
GGATCAA1181550.055.528679
GTGGGAT1302800.050.017196
CAGTGGG1423850.045.9110574
GGGATCA1788150.036.6228838
AGTGGGA1967950.033.031015
AAGCTGT1418550.025.1188661
TGGGATC2666400.024.5382447
GGGGAAA7144100.022.82109825-29
TGGGGAA5868150.022.68440625-29
GGGGGAA3175200.022.43532625-29
TGGGGGA3359900.021.41928725-29
Produced by FastQC (version 0.11.5)