FastQCFastQC Report
Mon 20 Mar 2023
SRR6503021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78947169
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7552170.9566106163984169No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5631140.7132795350774389No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5494890.6960211581494455No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3191430.40424882113252214No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1666770.2111247333010763No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1015200.1285923248242125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1366600.0143.83021
GATAGAC1035150.0142.50451
GCGAGTA2103100.0142.167881
ATGAATC2282600.0142.11592
TTCACGC2717600.0142.104491
ATCCGTC2342350.0141.874792
TAGCGAC1399850.0141.556762
TCACGCA2729000.0141.401412
AGAGATC2992600.0141.150632
AGCACCT1266700.0140.703741
CAGATTC1555950.0139.84822
CGAGTAA2131450.0139.838182
AGCCATG967200.0139.701491
CACGCAT1326700.0139.680663
AGTTAGC841700.0139.661352
GTACGCA791400.0139.61481
AATCCGT2380050.0138.907121
TAAGGTC1295650.0138.871752
AGCGACT683950.0138.704933
ATAGACA1068350.0138.524192
Produced by FastQC (version 0.11.5)