FastQCFastQC Report
Mon 20 Mar 2023
SRR6503016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105428296
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17825051.690727316696838No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7658760.7264425482130528No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1696010.1608685774452809No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1152430.10930936415779688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3795900.0143.260851
AGTGGTC5038400.0142.360241
ATGCCTA2004950.0141.989821
CGCATAC3557200.0141.837111
AACATCG6050350.0141.823322
CGAACTT1957100.0141.323531
GAGCTGA2363200.0141.1421
AGAGTCA1272900.0141.112991
CTCAATG2570800.0141.106031
ACATCGT3102000.0140.96383
CCGTGAG3354600.0140.63921
GCATACA3582350.0140.627062
ACTTCGA1536050.0140.603942
ACATCGA2016400.0140.441883
GTGGTCA5126500.0140.369862
CAAGCTA1531150.0140.362612
AGCTGAA2337400.0140.309072
AGTCACT1131100.0139.935241
AAACATC6174550.0139.763691
CGTGAGA3375200.0139.74732
Produced by FastQC (version 0.11.5)