FastQCFastQC Report
Mon 20 Mar 2023
SRR6503013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82837614
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1102891513.313897476573866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15628561.8866501876792348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA7835580.9458963895314513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6381170.7703227666600827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4993150.6027636189521345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3128880.3777124725007169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC2669560.3222642313188789No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2645890.3194068337120381No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1853980.22380895712423585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1626430.1963395517403483No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1547990.18687042338037405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1431720.17283452900997365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1370040.16538863613333915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1250640.15097489408615752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1234990.14908565570225138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1126800.1360251636412415Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT1055350.12739985485337615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1040380.12559270478263654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1013370.122332108696419No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA987180.11917050145843168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT980310.11834116805923454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC894840.1080233914028475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA893020.10780368444701943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTAAAAAAAAACCAACC828890.10006203220677022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT89594450.087.127361
AGTGGTA92354100.084.709735
AGCAGTG92488400.084.3494952
GTATCAA92823900.084.236229
GGTATCA94128350.083.002278
CAGTGGT93989900.082.987574
GCAGTGG94620700.082.401343
GTGGTAT96256450.081.274116
TGGTATC96148700.081.249487
GGTATAA456150.026.2142948
TGGTATA474200.025.4290127
ATGGGGG5034250.024.20561425-29
ATGGGGT2815650.023.22056625-29
ATGGGAT6038600.022.48730325-29
ATGGGAC3580650.022.32171625-29
ATGGGGC2975400.022.0691925-29
AGGAGTG451350.022.0686932
GGGGAAA3910700.021.69934725-29
TGGGGGA2322000.021.62020925-29
CATGGGG18893600.021.60241125-29
Produced by FastQC (version 0.11.5)