FastQCFastQC Report
Mon 20 Mar 2023
SRR6503011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64284328
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8851611.3769468042039732No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7518811.1696178888266515No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT804090.125083364019921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA3375250.0142.850272
ACGTATC1822450.0142.575911
CTCAATG3734300.0142.44171
AACGTGA2390900.0142.260361
CGAACTT2456400.0142.174881
ACGTGAT2396400.0142.092062
CAAGCTA1869750.0142.007572
AACATCG2648650.0141.982962
CGCATAC1910600.0141.972811
GAACTTA2466750.0141.745942
CACTTCG3401150.0141.248781
GCATACA1925100.0141.214162
ACAAGCT1882400.0141.046371
GTCACTA1210300.0140.772672
AGTCACT1213100.0140.657181
GACTAGT1586900.0140.612611
TCAATGA3792950.0140.460372
AGAGTCA929250.0140.435031
ATGCCTA924100.0140.093931
GAGTCAA930450.0140.023242
Produced by FastQC (version 0.11.5)