FastQCFastQC Report
Mon 20 Mar 2023
SRR6503011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64284328
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1016973715.819932036934414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15654312.4351674019210403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7792771.212234807836834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA7263801.1299488111628078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6492671.009992668819685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4480010.6969054728237962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1491810.2320643376718506No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1462770.22754690692263282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1252120.19477842251069344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1226920.19085833797624827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1130690.17588890405761107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA999780.15552468713680884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA961590.14958389236020325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA806780.125501817488082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC806430.12544737186954805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG801490.12467891085366871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA799870.12442690541931153No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA787750.12254153142893552No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT695950.10826122348202817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG683440.1063151815167143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA674470.10491981809314394TruSeq Adapter, Index 2 (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT670500.10430224922005873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT77031550.077.713661
AGCAGTG78898450.075.888772
AGTGGTA79196250.075.6396265
GTATCAA79275800.075.584639
GGTATCA80405250.074.416258
CAGTGGT80528850.074.346274
GCAGTGG81311050.073.588333
GTGGTAT81739050.073.290096
TGGTATC81668250.073.2626657
GGTATAA330800.033.3218
TGGTATA337450.032.8134967
GCAGTTG383700.026.1767923
CAGTTGT403800.024.9798564
CGAACTT42800.024.4093721
CATGGGG12841350.024.31811525-29
TGTATCA412850.024.2050238
GTTGTAT413650.024.106546
ATGGGGG4300400.023.81932425-29
ATGGGGT2131850.023.7720625-29
GGGGAAA3542750.022.87969425-29
Produced by FastQC (version 0.11.5)