FastQCFastQC Report
Mon 20 Mar 2023
SRR6503010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47288673
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9650332.040727596648779No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2347990.4965227085141509No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2175900.460131329969864No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG860390.18194420469358488No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA726600.1536520172600318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1196950.0142.159321
TAGCGAC1196100.0141.723832
AGAGATC1578850.0140.544042
AGTTAGC1139500.0140.27892
ATCCGTC1553250.0140.045062
GATAGAC788900.0139.862141
TCACGCA1475650.0139.837482
CTAACGA1760800.0139.24472
CTTCACA3472750.0138.610292
GCCAAGA1379700.0138.544511
GCCACAT1158050.0138.2911
AGCGACT588750.0138.071183
CCGACAA849650.0137.919531
TAACGAT965400.0137.552433
TCGTAGA935450.0137.430922
CACGCAT736350.0137.31643
CCAAGAC1399750.0137.193332
GAGATCT931700.0137.18543
CGACAAC856050.0137.175632
GGCTTCA1613100.0136.849852
Produced by FastQC (version 0.11.5)