FastQCFastQC Report
Mon 20 Mar 2023
SRR6503009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41120204
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6000801.459331281527689No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1865170.45358967577106374No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1086860.2643128910547234No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT539680.13124448507113437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA1456200.0141.34152
ACTTCGA1041750.0140.985082
ACAAGCT1461100.0140.416961
ACTAGTA1863850.0140.196142
AACGTGA918800.0140.104981
CGAACTT997050.0140.050541
ACGCTTA913600.0139.81272
GACTAGT1863250.0139.496671
AGAGTCA719800.0139.386721
GAACTTA998250.0139.373282
ATGCCTA778200.0139.282471
ACGTGAT927800.0139.102432
CGTGAGA1233700.0138.891892
AGCTGAA836550.0138.808382
GAGTCAA719950.0138.771382
CGCATAC836700.0138.391431
GATCGCA1226050.0138.353352
ACCGAGA703100.0138.098662
GCATACA833300.0138.026232
ATGTGAC1147200.0137.981585
Produced by FastQC (version 0.11.5)