FastQCFastQC Report
Mon 20 Mar 2023
SRR6503006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66781171
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19143212.8665580003082005No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6150670.9210185907042571No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5785850.8663894198560849No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4767800.71394375519411No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1934390.2896609884244168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1370900.0144.021551
TATCAGC1512400.0142.924131
TAGCGAC1395800.0141.830932
AGTTAGC1683750.0141.625142
AGAGATC2776550.0141.276952
AGGCTAA2394000.0141.2131
GGCTAAC2412700.0140.841052
ATCAGCA1539000.0140.191792
ATCATTC1777750.0140.156631
ACCTTAC2665250.0139.965042
GCGAGTA1038100.0139.952381
GAGTTAG1707400.0139.418231
ATGAATC798000.0139.110532
GCTAACT1219900.0138.96053
GATAGAC396850.0138.49591
TCACGCA1095950.0138.114962
CACCTTA2695850.0138.055651
GAGATCT1651800.0137.917273
GTCGTAG608800.0137.341581
CCGACAA1818450.0137.26431
Produced by FastQC (version 0.11.5)