FastQCFastQC Report
Mon 20 Mar 2023
SRR6503004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103723069
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3633130735.02721945105577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35079423.3820268083274705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAGGTGATAAAAAAAAAA14852961.4319823105118497No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8398000.809655950307448No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7791590.7511916177489889No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT6669990.6430575246476751No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6499980.6266667639770667No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5974870.5760406105993644No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5090770.49080402740493534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT5031810.48511966031394615Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4883410.4708123320184442No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4585980.44213693676958205No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4344320.4188383588996966No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4270450.41171651023939526No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4214330.4063059491616084No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA4169030.4019385504298952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC3758020.3623128428643005No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3673270.35414204722384374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3665060.35335051646032567No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3657340.3526062268751419No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3284090.31662098235832187No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3136610.3024023517854066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3006450.2898535522507534No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2993240.28857996864709046No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2983530.287643822031529No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2929610.2824453642034059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC2880970.27775595417447585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2846090.27439315356162475No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2581070.24884242482258212No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA2449290.236137440167722No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2281960.2200050598194313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2200060.21210903429785713Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2078030.2003440526812796No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2050090.19765034141054966No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1747130.1684417957204872No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1745040.1682402976333066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1696150.16352678496236936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1687970.16273814651589225No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1572520.15160754643694546No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1472160.14193178182955615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTAAAAAAAAACCAACC1404470.1354057504796739No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1374520.13251825396720568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1319250.12718964187224346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1289850.12435517117219122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1207950.11645914565061703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1194800.11519134668103582No Hit
AAGCTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1170830.11288038536538096No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1160640.11189796167716556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT222953550.042.1091581
AGCAGTG232874700.040.3366622
AGTGGTA233950300.039.945485
GTATCAA236436100.039.8189139
GGTATCA238330700.039.4867178
GCAGTGG238317900.039.416733
CAGTGGT237204450.039.395074
TGGTATC241243450.039.0075347
GTGGTAT241361350.038.9641886
AAGCTGT1044650.033.5608251
AGTGGGA1907500.027.4521775
CAGTGGG1947100.027.1466394
GGATCAA1989200.026.8214959
GTGGGAT2403950.022.1505936
TGTGGTA1571150.021.5927985
AGCTGTG1779900.019.706622
GGGATCA2868050.018.620638
ACACTCG161350.017.77237530-34
ACGTTTA273900.017.71024555-59
CGCATTC272450.017.08233885-89
Produced by FastQC (version 0.11.5)