FastQCFastQC Report
Mon 20 Mar 2023
SRR6503000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111477826
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3913518835.105804808213605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA60364265.414911840853445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13909111.2477019420884652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAGGTGATAAAAAAAAAA8144030.7305515627834364No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7813160.7008712207932724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6531020.5858582136325479No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6072390.5447172965141964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5614000.5035979083409825No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5408000.48511889709797534No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4861460.43609210678363963No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4565130.40951013881451187No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3863780.34659628184711816No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3523800.3160987369811105No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3300320.29605170090058985No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3176520.28494635336717095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3046180.27325434207875565No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2897410.2599090872116577No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2763190.24786902464351968No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2538640.2277260053492611No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2394610.2148059471486285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2367200.21234716220605165No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2346070.2104517179945723No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2310670.2072761985867934No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2241380.2010606127177256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2234740.20046497856892184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2074120.18605673203566062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2050840.18396842435732466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2003570.179728119204621No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1984750.17803989109008997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1972890.17697600238454597No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1931220.1732380392850503No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA1807450.16213538286977358No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1705760.15301338940714543No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1679750.15068018997787058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1664170.14928260262269558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1657890.14871926189159806No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGG1582010.14191252707063018No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1510140.1354655050413344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1484980.1332085539594215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1450320.1300994154658165No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG1396180.12524284425855237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1336790.11991532737640577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG1327280.11906224292533296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1316470.11809254335476546No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1307770.11731211909353166No Hit
AAGCTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1280430.11485961342661993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1258510.11289330310406305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1217390.10920467717050744Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1203100.10792280789544641No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAG1197860.10745275925994466No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1183630.10617627222116799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA1175990.10549093413429142No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1132280.10156997500112713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1129970.10136275890417884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA1573150.054.2255489
CAGTGGG1729550.049.2970854
AAGCAGT254755600.048.1296961
GTGGGAT1782550.047.867676
AGTGGGA1807800.046.5461655
GTATCAA266161700.045.9449129
AGTGGTA266147100.045.765265
AGCAGTG268157850.045.7334332
GGTATCA271484300.045.0363168
GCAGTGG276394800.044.386243
CAGTGGT275966500.044.1696554
TGGTATC279763650.043.7327047
GTGGTAT279860200.043.7095956
GGGATCA1954150.043.6605768
AAGCTGT1597200.031.3803961
TGGGATC3287200.025.9900467
GACTGGA309250.021.5565283
AGCTGTG2386100.020.9591462
TGACTGG329750.020.3555872
TGTGGTA2421300.020.0054875
Produced by FastQC (version 0.11.5)