FastQCFastQC Report
Mon 20 Mar 2023
SRR6502998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94553380
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1874065619.820186227081464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12663221.339266771848875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5104190.5398209984666862No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4110510.43472903877153835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3852040.4073931571774589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3235430.34218025838949384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2297830.24301933997494327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2013030.21289878796506267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1915950.20263157171113289No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1871940.19797705803853866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1607400.1699992110276756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1592130.16838425025102222No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1499060.1585411330615574No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1350340.1428124515485327No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1227670.12983882754905218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1226270.12969076303776766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1206160.12756392209353065No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1202130.12713770782176165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1183340.12515047055959289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1179790.12477502126312143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1143390.12092534396972376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1111960.11760129569138619No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA997840.1055319228143933No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT991830.1048963030195219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT122090100.067.6680761
AGCAGTG128780350.064.260362
AGTGGTA128750400.064.2490845
GTATCAA129823250.063.758919
GGTATCA132235300.062.6078878
CAGTGGT133121750.062.2119984
GCAGTGG133822000.061.849543
GTGGTAT135194500.061.240886
TGGTATC135776250.061.0143437
CGACCTA353200.022.92139445-49
AAACCGC858500.021.84320385-89
GAAACCG883850.021.65502285-89
ATGGGGC4094150.021.48925225-29
GATAAGT898150.021.38024995-99
TACGCAC380700.021.33049250-54
CGCGAAA234000.020.7041590-94
ATGGGGG4576050.020.61223825-29
AGTTCAG564200.020.4141069
ATGGGCT2837600.019.91017325-29
GGGCTTT1707850.019.813625-29
Produced by FastQC (version 0.11.5)