FastQCFastQC Report
Mon 20 Mar 2023
SRR6502995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81552331
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22973102.8169765006471734No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9156701.122800524242526No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT6184120.758300826496302No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2307270.282918951758718No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1195960.14664939497560162No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT861170.1055972268898114No Hit
CAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT830360.10181928460144198No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG828790.10162677017778927Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC4106600.0142.126171
AACGTGA1920850.0141.479421
ACGTGAT1934250.0141.010152
CAAGCTA2083150.0140.970932
ATGCCTA1344000.0140.229051
AACATCG1909600.0139.889592
ACTTCGA1040450.0139.203142
ACAAGCT2111100.0139.19021
AAGACTA1448450.0139.035932
ACGCTTA1049600.0138.929372
CTCAATG2305750.0138.873081
ATCCTGT2964300.0138.731741
CAATGGA3277600.0138.726411
TGCCTAA1355200.0138.599322
CAAGACT1462350.0137.736131
CCGAAGT1090650.0137.550461
TCCTGTA2987050.0137.54172
CGAAGTA1090900.0137.39192
AAGCTAT1070000.0137.185363
GTCACTA825100.0136.862672
Produced by FastQC (version 0.11.5)