FastQCFastQC Report
Mon 20 Mar 2023
SRR6502993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97206681
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17397031.789694887329812No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6440570.6625645412170795No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2144300.2205918336004086No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1570550.16156811279257646No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1152840.11859678657272538No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1032120.10617788709399512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGC6255950.0142.82031
CCGAAGT3740850.0142.77421
AGTCACT3432100.0142.763961
AGAGTCA2575100.0142.753651
ATCCTGT6080700.0142.502431
CGAAGTA3755550.0142.129142
GATCGCA6276500.0142.010622
GTCACTA3445950.0141.988542
CGCATAC2236800.0141.664341
GAGTCAA2598500.0141.30112
ATCGCAT3534450.0140.6843
AACGTGA1186750.0140.555371
CAATGGA4549000.0140.403031
TCCTGTA6174850.0140.361482
CAAGACT2259950.0140.229251
AAGACTA2256000.0140.1212
ACGTATC1598500.0140.060321
ATCGCAA2083750.0139.968813
ACGTGAT1195250.0139.51932
GCATACA2263500.0139.21482
Produced by FastQC (version 0.11.5)