FastQCFastQC Report
Mon 20 Mar 2023
SRR6502992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90360619
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2941483732.55271746201739No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA74070558.197215869005944No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8264860.91465287549657No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8067090.8927661285720055No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7629600.8443501255784891No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7327010.8108631925153147No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7268770.8044179068760031No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT5842650.6465925161490981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC5623470.6223363742118676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5424600.600327892840132No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5369850.5942688374013906No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5106370.5651101172735437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT4891960.5413818601663187Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4796870.5308584705467766No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4628180.512189939734698No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA4471770.49488040802376526No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4405880.4875885146382187No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4273530.47294164728995497No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4269510.47249676321938433No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4227710.46787085422688396No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3993460.4419469503634099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA3503810.3877585212204002No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3405100.3768345146019861No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT3252750.35997429366879397No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC3244310.3590402584559541No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3072560.3400330845453814No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3047420.3372508990891264No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3006820.3327577913117218No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2887230.3195230435506423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG2698860.29867657281099413No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2615320.2894313948867482No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2553670.2826087324612063No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2552840.2825168782874318No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG2334860.25839353756529715No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2328930.2577372782273658No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA2321920.2569614977958484No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2202190.24371125655967452No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2177650.24099547171096736No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA2082220.2304344550804815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA1910900.21147486827198472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1842060.20385650523266113No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG1765300.19536165417370593No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1746020.19322798131783495No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACCGATGT1730190.1914761119553641TruSeq Adapter, Index 2 (100% over 32bp)
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG1700990.1882446157213686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1600790.17715571426087728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA1567150.1734328535310277No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1558740.17250213834856531No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAGTGGTATCAACGCAGA1498600.16584658411868558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1498290.16581227713811922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1470890.16277998272676727No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1455060.16102811336429645No Hit
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT1322250.14633033888357935No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA1297520.1435935271758154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1180870.13068414239172044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGG1173710.12989176180831608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAAAGCAGTGGTATCAACGCAGA1151660.12745153948093252No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1095050.12118664215879264No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1093920.12106158768124418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1082300.11977562924840078No Hit
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA1078880.11939714578537804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG1071020.11852729782650118No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1056970.11697241693308896No Hit
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT1025410.11347974497607193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1019390.11281352554700849Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA1016700.11251582948983561No Hit
AAGCTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG985630.10907738469564934No Hit
CAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC972770.10765419834054037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG960070.10624871881411081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGATCGGAAGAGCACACGTCT938050.10381181651710465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG937930.10379853639559508Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGCAAGCAGTGGTATCAACGCAGA926520.10253581817539342No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG911800.10090678993688611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT231210950.035.4056241
AGCAGTG242170850.033.822922
AGTGGTA245178350.033.2275625
GCAGTGG248811900.032.9214973
CAGTGGT249508600.032.6504064
GTATCAA252104450.032.5585069
GGTATCA255528900.032.1124578
GTGGTAT257384100.031.860376
TGGTATC258200050.031.7771957
AAGCTGT1090600.028.8654211
AGTGGGA1780000.026.6415215
GGATCAA2096100.023.0850729
CAGTGGG2245600.021.4767534
AGCTGTG1536000.020.4750082
GTGGGAT2426500.019.8602246
GGGATCA2516950.019.2254648
TGTGGTA1705000.017.9803165
GCTGTGG2255950.013.9139173
CTGTGGT2286050.013.4097444
CTACTTA81300.012.85377135-139
Produced by FastQC (version 0.11.5)