FastQCFastQC Report
Mon 20 Mar 2023
SRR6502991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76545831
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11950221.561184958590364No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3371040.44039498375816183No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2445860.3195288323409801No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1666960.21777280071595276No Hit
CATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGTTGATAC1056390.1380075160461711No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG985310.12872157596669112Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATAC774590.10119297026117595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG1603400.0141.978621
CATCAAG1382700.0141.668331
CAATGGA2507550.0139.802051
ATCAAGT1413950.0139.603162
ACATTGG1656300.0138.02971
CAAGACT1117850.0137.749191
TCAATGA1627650.0137.657642
AAGACTA1111400.0136.49212
TGCCTAA531450.0136.48372
CATTGGC1657200.0136.478332
ATGTGAC2530650.0136.120075
ATGCCTA550250.0134.194671
CGAACTT451400.0133.495571
AGACTAT582550.0132.439823
GATGTGA3808500.0132.140764
GCCTAAT288800.0131.816773
AATGGAA2651450.0131.754942
TCAAGTT916150.0131.03393
AATCTGA949800.0130.811252
ATTGGCA601300.0130.741363
Produced by FastQC (version 0.11.5)