FastQCFastQC Report
Mon 20 Mar 2023
SRR6502990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86352211
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1083324412.545415889814333No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19726862.284464957127734No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16954611.9634251171634736No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15131761.7523303485535535No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9683801.121430463430751No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC6624580.7671581217532462No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA5271340.6104464424194072Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2557900.29621708238599703No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA2212440.2562111582759589No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1740870.20160109160378073No Hit
CCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATA1706010.1975641364874838No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1640330.18995807762235525No Hit
CCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATAC1533580.17759591587064286No Hit
AGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1513200.1752358141704096No Hit
CCATGTACTCTGCGTTGATACCACTGTTCCCATGTACTCTGCGTTGATAC1448430.1677351376677547No Hit
NCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1409970.16328128529332042No Hit
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT1330230.15404701102557755No Hit
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA1280930.14833783468497408No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1027950.11904153791730937No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1016780.11774799836914425No Hit
CCATGTACTCTGCGTTGATACCACTGCTTTCCCATGTACTCTGCGTTGAT992470.1149327838287777No Hit
CCATGTACTCTGCGTTTATACCACTGCTTCCCATGTACTCTGCGTTGATA962890.11150727802441561No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGAT923520.10694804328750772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG7598900.0143.816851
AGATGTA3743950.0141.990751
ATGAATC1204800.0139.600982
TTGGCGT6954400.0137.192892
AACTCAC1448700.0136.807361
TAGGATG1373750.0136.642941
TCACGCA1200800.0136.546662
TATCAGC1216450.0135.943531
GATGTAC3917750.0135.820652
CTAACGA609450.0135.351822
AGGATGA1378700.0134.807052
TGGAACA1823200.0134.313541
AGCCATG989550.0134.231351
TTCACGC1232550.0133.487371
AGTTAGC382150.0133.437162
ATTGGCT659700.0133.195921
TCTGTCA1058650.0133.181052
GCGTGAC1757000.0133.148355
ATGTTGC830650.0133.083792
TGGCGTG6081050.0132.5763
Produced by FastQC (version 0.11.5)